Vanderbilt Pathology Laboratory Services
Test Directory
BONE MARROW ENGRAFTMENT (RFL)
Department: Molecular DiagnosticsTest Synonym(s): Identity Testing, RFL, RFLP DNA Analysis
CPT Codes: 81265, 81266, 81267
Methodology: Analysis of DNA Polymorphisms for identification of Host and Donor Cells Post Transplantation (Identity/Discrimination Between Samples) by fluorescent PCR and evaluated by capillary electrophoresis
Tube Type: Lavender top (EDTA)
Specimen: Blood or bone marrow, 5-10 mg Chorionic Villi, 2-3 T-25 flasks-cultured cells, paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10ìm thick with accompanying before and after cut H & E slides); purified DNA (1 ìg)
Alternate Specimen: Yellow top (ACDH) for whole blood or bone marrow
Pediatric Requirements: 0.5 mL whole blood or bone marrow
Volume: 4 mL (whole blood or bone marrow)
Minimum Volume: 0.5 mL whole blood or bone marrow
Temperature: EDTA and ACDH tubes: room temperature or refrigerated, Chorionic Villi: refrigerate, flasks (cultured cells): room temperature or refrigerate, purified DNA: refrigerate or freeze, paraffin block: room temperature
Stability: EDTA and ACDH tubes - Room temperature: 2 days Refrigerated: 3 days; Purified DNA - indefinitely @ -70°C; Paraffin block - indefinitely; Chorionic villi and cultured cells - call Molecular Diagnostics Lab at 615-343-8121
Reasons for Rejection: Decalcification (paraffin embedded tissue). Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
Days Performed: Monday - Friday
Times Performed: Batched
TAT: 3 - 5 days
Significance: Analysis of DNA independently segregating polymorphic sites and 1 gender specific marker for the identification of Recipient versus Donor Cells Post Transplantation
Special Instructions: Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission or if patient has received a transfusion in the prior 2 weeks. Chorionic Villi must be free of maternal cells. Cultured cells - backup culture must be maintained in the event that additional DNA is required.
Click here to print the Molecular Diagnostics requisition.
Search Tests
Search Help
Search Options for the Test Directory:
1. Enter the test name in the Keyword search field.
Hint: You can also enter any part of the test name to expand the search.
(Example: you can type "west" or "sed" or "rate" to find Westergren Sedimentation Rate)
2. Enter the Vanderbilt Test Code, if known.
3. Choose one of the letter boxes to display a list of all test names and keywords starting with that letter.
If you are unable to find a test in our directory, please contact VPLS at
1-800-551-5227 or e-mail emily.k.thompson@vanderbilt.edu.
