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Vanderbilt Pathology Laboratory Services

Test Directory



ADENOVIRUS BY PCR (ADV)

Department: Molecular Infectious Disease
Test Synonym(s): ADV, Adenovirus DNA
CPT Codes: 87798
Methodology: PCR (Polymerase Chain Reaction)
Reference Range: Not detected
Tube Type: Swab in Universal Transport Medium, Sterile container
Specimen: Nasal washing, nasal secretions, nasopharyngeal swabs
Alternate Specimen: Need Medical Director Approval
Pediatric Requirements: Nasal washing, nasal secretions, nasopharyngeal swabs
Volume: 1.0 mL
Minimum Volume: 0.5 mL
Temperature: Refrigerated: 3 days; -80C: longer than 3 days
Stability: Refrigerated: 3 days; -80C: longer than 3 days
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday, Wednesday and Friday
Times Performed: Once per day on Monday, Wednesday and Friday (batched)
TAT: 1 - 3 days
Significance: Human adenoviruses (AdV) are ubiquitous and responsible for a variety of diseases. Most symptomatic infections are mild and self-limited. Febrile and/or respiratory tract infections in young children are a common clinical presentation. Other AdV-associated diseases include conjunctivitis, myocarditis, and diarrhea. Serious, and sometimes fatal, disseminated infections may occur in immunocompromised hosts, especially recipients of hematopoietic and solid-organ transplants; affected organ-systems include pulmonary, gastrointestinal, hepatobiliary, urinary, and CNS. Culture has historically served as the "gold standard" for diagnosis of AdV infection. Though a sensitive method of diagnosis, culture may require days to weeks for viral isolation. Immunofluorescence and other immunodiagnostic assays using direct antigen detection are more rapid than culture, but lack sensitivity, and serology does not have a significant role in routine clinical management of AdV infections. PCR offers a very sensitive and specific method of AdV detection in immunocompetent adults.
Special Instructions: Transport on wet ice. Click here to print the Molecular Diagnostics requisition.

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