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Vanderbilt Pathology Laboratory Services

Test Directory



CMV QUANTIFICATION (CMQ)

Department: Molecular Infectious Disease
Test Synonym(s): Cytomegalovirus, CMQ, CMV DNA quant
CPT Codes: 87497
Methodology: PCR (Polymerase Chain Reaction)
Reference Range: Not detected
Tube Type: Lavender top (EDTA) - 4.0 mL
Specimen: Plasma spun, separated from RBCs and refrigerated within 6 hours of collection
Alternate Specimen: Need Medical Director approval
Pediatric Requirements: 1.5 mL EDTA Plasma
Volume: 1.5 mL EDTA Plasma
Minimum Volume: 1.5 mL EDTA Plasma
Temperature: Refrigerated: 7 days; Frozen: 6 weeks (preferably -80C)
Stability: Refrigerated: 7 days; Frozen: 6 weeks (preferably -80C)
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday - Friday
Times Performed: Batched
TAT: 1 - 3 days
Significance: CMV infection in immunocompetent individuals is usually asymptomatic; however, primary infection is associated with latency in a wide range of cells and tissues, including leukocytes. Immunocompromised individuals, including transplant patients and AIDS patients, are at risk for reactivation of latent CMV infection and development of severe disease. Clinical manifestations of CMV disease in immunocompromised hosts include fever, hematological abnormalities, retinitis, gastroenteritis, hepatitis, encephalitis, esophagitis, enterocolitis, pancreatitis, and pneumonia. Studies in transplant recipients have shown an association between CMV viral load in blood and risk of developing CMV disease. Similarly, increases in viral load over time have been associated with worse clinical outcomes. Quantitative PCR assays allow for diagnosis of active CMV infection, monitoring progression of disease, and assessing the virologic response to antiviral treatment.
Special Instructions: Transportation of whole blood, room temperature or refrigerated. Transportation of plasma, refrigerated or frozen. Analytical measurement range: 137-3,000,000 CMV DNA IU/mL
Click here to print the Molecular Diagnostics requisition.

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