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Vanderbilt Pathology Laboratory Services

Test Directory



EBV DETECTION BY AMPLIFIED PROBE (EBD)

Department: Molecular Infectious Disease
Test Synonym(s): Epstein Barr Virus, EBD
CPT Codes: 87798
Methodology: PCR (Polymerase Chain Reaction)
Reference Range: Not detected
Tube Type: CSF container - 1.0 mL, lavender top (EDTA) - 4.0 mL
Specimen: CSF or whole blood
Alternate Specimen: Need Medical Director approval
Pediatric Requirements: CSF container - 1.0 mL, lavender top (EDTA) - 1.0 mL
Volume: 1.0 mL
Minimum Volume: 0.5 mL
Temperature: Refrigerated: 1 week; -80C: longer than 1 week
Stability: Refrigerated: 1 week; -80C: longer than 1 week
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday, Wednesday and Friday
Times Performed: Once per day on Monday, Wednesday and Friday (batched)
TAT: 1 - 3 days
Significance: Infection with EBV occurs early in life. Usually, infection in children is asymptomatic or mild and may be associated with minor upper respiratory tract illness. However, EBV also is the causative agent of infectious mononucleosis and a variety of neoplastic diseases (e.g., non-Hodgkin B-cell and CNS lymphomas, rare T-cell lymphomas, and nasopharyngeal carcinoma). These disorders are mostly lymphoid in nature and concentrated in immunocompromised patients, though individuals with normal immunity may develop EBV-associated neoplasms. In addition, encephalitis is a recognized sequelae of EBV infection, particularly in immunocompromised populations such as AIDS and organ-transplant patients. Convenient cell culture systems for isolation of EBV from blood or CSF are not available. PCR detection of EBV DNA in these specimen types is the preferred laboratory diagnostic approach to disease caused by this virus.
Special Instructions: Click here to print the Molecular Diagnostics requisition.

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