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Vanderbilt Pathology Laboratory Services

Test Directory



HEPATITIS B DNA QUANTIFICATION BY PCR (HBD)

Department: Molecular Infectious Disease
Test Synonym(s): HBV DNA by PCR, HBV DNA quant, HBD
CPT Codes: 87517
Methodology: PCR (Polymerase Chain Reaction)
Reference Range: No HBV DNA detected.
Tube Type: Lavendar top (EDTA) - Two full tubes
Specimen: Plasma spun and separated from RBC within 6 hours of collection
Alternate Specimen: Red top SST (clot activator with gel). Any other needs Medical Director's approval.
Pediatric Requirements: 1.5 mL EDTA plasma
Volume: 1.5 mL EDTA plasma
Minimum Volume: 1.5 mL EDTA plasma
Temperature: Room temperature: 1 day; Refrigerated: 5 days; Frozen (preferably -80C): longer than 5 days
Stability: Room temperature: 1 day; Refrigerated: 5 days; Frozen (preferably -80C): longer than 5 days
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Friday
Times Performed: Batched
TAT: 1 - 7 days
Significance: HBV is one of the leading causes of chronic hepatitis, cirrhosis, and hepatocellular carcinoma. More than 2 billion people are infected worldwide, and this virus is responsible for 1 million deaths annually. In developed countries, HBV is a blood-borne viral infection transmitted via sexual activity, injection-drug use, or occupational exposure. Almost all vertical transmission (mother to infant) of HBV has been eliminated in the United States due to the institution of an aggressive HBV vaccination program and the immunoprophylactic treatment of infants born to HBsAg positive mothers. Chronic HBV patients may go on to develop more severe conditions such as cirrhosis, hepatocellular carcinoma, liver failure, and other complications. There have been recent advances in antiviral therapy with the development of new nucleoside analogues. These developments support the need for HBV viral quantification in treatment monitoring. Quantitative PCR is the standard method of viral load determination.
Special Instructions: Transportation of whole blood, room temperature or refrigerated. Transportation of plasma, refrigerated or frozen. Analytical measurement range: 20 - 170,000,000 HBV DNA IU/mL.


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