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Vanderbilt Pathology Laboratory Services

Test Directory



HEPATITIS C VIRUS GENOTYPING BY PCR AND SEQUENCING (HC5)

Department: Molecular Infectious Disease
Test Synonym(s): HC5, Hep C typing, HCV typing, HCV GT
CPT Codes: 87902
Methodology: PCR (Polymerase Chain Reaction)
Reference Range: No HCV RNA detected
Tube Type: Lavender top (EDTA) - 4.0 mL
Specimen: Plasma spun and separated from RBC within 6 hours of collection
Alternate Specimen: Red top SST (clot activator with gel). Other specimen sources need Medical Director approval.
Pediatric Requirements: 1.5 mL EDTA plasma
Volume: 1.5 mL EDTA plasma
Minimum Volume: 0.5 mL EDTA plasma
Temperature: Room Temperature: 1 day; Refrigerated: 5 days; Frozen: longer than 5 days (preferably -80C)
Stability: Room temperature: 1 day, Refrigerated: 5 days, Frozen: longer than 5 days (preferably -80C)
Reasons for Rejection: Alternative specimen sent without Medical Director approval or improper storage
Days Performed: Thursday
Times Performed: Once every Thursday (batched)
TAT: 1 - 7 days
Significance: "The World Health Organization estimates that 3% of the global population has been infected with HCV, translating to >170 million chronically infected persons at risk of progression to cirrhosis and/or hepatocellular carcinoma. HCV exhibits extensive genetic diversity; six major genotypes and dozens of subtypes have been distinguished. Viral genotyping studies should be performed as a prerequisite to treatment initiation since genotype data guide selection of an optimal therapeutic regimen. Clinicians rely on knowledge of HCV genotype for choosing a therapeutic management strategy that maximizes probability of cure while minimizing adverse effects of antiviral medications.
Special Instructions:
Click here to print the Molecular Diagnostics requisition.

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