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Vanderbilt Pathology Laboratory Services

Test Directory



HIV - 1 VIRAL LOAD BY PCR (HNA)

Department: Molecular Infectious Disease
Test Synonym(s): HNA, HIV RNA quant, HIV viral load
CPT Codes: 87536
Methodology: PCR - Polymerase Chain Reaction
Reference Range: No HIV-1 RNA detected
Tube Type: Lavendar top (EDTA) - Two full tubes
Specimen: Plasma spun and separated from RBC within 6 hours of collection.
Alternate Specimen: Need Medical Directory approval
Pediatric Requirements: 1.5 mL EDTA plasma
Volume: 1.5 mL EDTA plasma
Minimum Volume: 1.5 mL EDTA plasma
Temperature: Room temperature: 1 day; Refrigerated: 5 days; Frozen: (preferably -80C): longer than 5 days
Stability: Room temperature: 1 day; Refrigerated: 5 days; Frozen (preferably -80C): longer than 5 days
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday - Friday
Times Performed: Batched
TAT: 1 - 3 days
Significance: "HIV is the etiologic agent of acquired immunodeficiency syndrome (AIDS). Quantitative measurements of HIV viremia have shown that higher virus levels are correlated with increased risk of clinical progression of HIV disease and that reductions in virus levels are associated with decreased risk of clinical progression. Virus in peripheral blood can be quantified by measurement of viral RNA in plasma using nucleic acid amplification technologies. Real-time PCR assays have emerged as field-standard methods of sensitive and accurate HIV-1 RNA quantification in plasma.
Special Instructions: Transportation of whole blood, room temperature or refrigerated. Transportation of plasma, refrigerated or frozen. Analytical measurement range: 40 - 10,000,000 cpy/mL.
Click here to print the Molecular Diagnostics requisition.

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