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Vanderbilt Pathology Laboratory Services

Test Directory



HIV - 1 VIRAL LOAD BY PCR (HNA)

Department: Molecular Infectious Disease
Test Synonym(s): HNA, HIV RNA quant, HIV viral load, HIV 1 ultra quant
CPT Codes: 87536
Methodology: PCR - Polymerase Chain Reaction
Reference Range: No HIV-1 RNA detected; limits of detection: 40 - 10,000,000 copies/mL
Tube Type: Lavendar top (EDTA) - Two full tubes
Specimen: Plasma spun and separated from RBC within 6 hours of collection.
Alternate Specimen: Lavender top (EDTA)
Pediatric Requirements: Need Medical Director approval
Volume: 1.5 mL EDTA plasma
Minimum Volume: 1.5 mL EDTA plasma
Temperature: Room temperature: same day, refrigerated: overnight, frozen: longer than overnight
Stability: Refrigerated: overnight, Frozen if longer than overnight
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday - Friday
Times Performed: Batched
TAT: 48 hours
Significance: HIV is the etiologic agent of acquired immunodeficiency syndrome (AIDS). Quantitative measurements of HIV viremia in the peripheral blood have shown that higher virus levels may be correlated with increased risk of clinical progression of HIV disease, and that reductions in plasma virus levels may be associated with decreased risk of clinical progression. HIV viral load and drug treatment response are closely linked. With successful drug therapy the viral load decreases and with failure the load increases.
Special Instructions: Plasma preparation tube must be centrifuged and refrigerated within six (6) hours of collection. EDTA plasma from a lavender top tube must be separated from the red cells and refrigerated within six (6) hours.
VANDERBILT INPATIENT UNITS: Contact MIDL (6-6435)for PPT Tubes.
VPLS CLIENTS: Contact VPLS (800-551-5227, x3) for PPT Tubes.
Click here to print the Molecular Diagnostics requisition.

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