HIV - 1 GENOTYPING (HVG)
: Molecular Infectious Disease
: HVG, HIV resistance testing
: PCR (Polymerase Chain Reaction)
: Resistance or resistant mutations reported
: White top PPT (EDTA with gel) or lavender top (EDTA)
: Need Medical Director approval
: 1.0 mL EDTA plasma
: 2.0 mL EDTA plasma
: 1.0 mL EDTA plasma
: Room Temperature: same day, Refrigerated: overnight, Frozen: longer than overnight
: Room temperature: same day, Refrigerated: overnight, Frozen: longer than overnight
Reasons for Rejection
: Alternative specimen sent without Medical Director approval
: Batched once per week
: 3-10 days
: Therapeutic failure for HIV infection has been closely linked with resistance to antiretroviral drugs. Mutations in the HIV-1 reverse transcriptase (RT) and protease targeted by antiretrovirals can confer drug resistance. Genotypic testing detects resistance-associated mutations in the RT- and protease-encoding genes. Resistance testing is recommended for (1) patients with acute HIV infection, (2) antiretroviral therapy (ART)-naive patients entering HIV care, (3) patients with virologic failure of ART, (4) patients with suboptimal suppression of viral load, and (5) HIV-positive pregnant women. (See Guidelines for the Use of Antiretoviral Agents in HIV-1-Infected Adults, Dept. of Health and Human Services, Oct. 14, 2011).
: Plasma preparation tube must be centrifuged and refrigerated within six (6) hours of collection. EDTA plasma from a lavender top tube must be separated from the red cells and refrigerated within six (6) hours.
VANDERBILT INPATIENT UNITS: Contact MIDL (6-6435) for PPT Tubes.
VPLS CLIENTS: Contact VPLS (800-551-5227, x3) for PPT Tubes.
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