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Vanderbilt Pathology Laboratory Services

Test Directory



MYCOBACTERIUM TUBERCULOSIS DETECTION BY PCR (TBD)

Department: Molecular Infectious Disease
Test Synonym(s): TBD, TB DNA PCR
CPT Codes: 87556, Paraffin embedded 83907, Concentration 87015
Methodology: PCR - Polymerase Chain Reaction
Reference Range: No M. tuberculosis DNA detected
Tube Type: Tissue in paraffin block
Specimen: Tissue
Alternate Specimen: CSF, urine and respiratory specimens submitted for TB PCR are sent to a reference lab. Please call the lab for specimen requirements of reference tests. Other specimen types require Medical Director approval; contact the lab prior to specimen collection for approval and collection instructions
Pediatric Requirements: Tissue in paraffin block
Volume: Paraffin block
Minimum Volume: Paraffin block
Temperature: Room temperature
Stability: Room temperature: same day, refrigerated: overnight, frozen: longer than overnight
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Weekly
Times Performed: Once per week
TAT: 72 hours
Significance: PCR for Mycobacterium tuberculosis complex (MTB) has already proved to be a useful tool for the diagnosis of tuberculosis infection. For histopathologic investigations, human tissue samples are mostly stored as formalin-fixed, paraffin-embedded blocks. The molecular amplification techniques can be used in DNA extracted from tissue blocks rule out MTB in tissues stained positive by acid-fast stain, thereby improving diagnosis.
Special Instructions: Other sources or samples may be acceptable for this test; please check with the lab, 615-936-6435.
Click here to print the Molecular Diagnostics requisition.

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