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Vanderbilt Pathology Laboratory Services

Test Directory



MYCOBACTERIUM TUBERCULOSIS DETECTION BY PCR (TBD)

Department: Molecular Infectious Disease
Test Synonym(s): TBD, TB DNA PCR
CPT Codes: 87556, Paraffin embedded 83907, Concentration 87015
Methodology: PCR - Polymerase Chain Reaction
Reference Range: No M. tuberculosis DNA detected
Tube Type: Sterile container; Paraffin block
Specimen: Formalin-fixed, paraffin embedded blocks; Fresh tissues
Alternate Specimen: Formalin-fixed tissues
Pediatric Requirements: Formalin-fixed, paraffin embedded blocks; Formalin-fixed tissues; Fresh tissues
Volume: 20 - 25 mg
Minimum Volume: 20 mg
Temperature: FFPE: Room temperature; Fresh tissue: -80C
Stability: FFPE: Room temperature; Fresh tissue: -80C
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday
Times Performed: Once per week
TAT: 2 - 15 days
Significance: Mycobacterium tuberculosis (MTB) is a globally distributed pathogen infecting one-third of the world's population. This organism ranks as the second most common infectious cause of death worldwide due to a single agent. MTB is best known for its pulmonary pathogenesis; however, virtually all organ-systems may be infected. Accurate diagnosis of MTB underpins critical treatment and containment measures to prevent morbidity, mortality, and transmission. MTB PCR has proven to be a useful tool for the diagnosis of infection. For histopathologic investigations, human tissue samples are typically stored as formalin-fixed paraffin-embedded (FFPE) blocks. Widening of the applicability of amplification techniques to tissue blocks can bring relevant improvements to the routine diagnosis of tuberculosis. This is particularly true when the pathogen fails to grow in culture and for those patients in whom MTB infection had not been clinically suspected and, therefore, not sought via culture. A FFPE tissue block may be the only available material in such situations, precluding culture due to inactivating effects of tissue fixatives. Extraction of total nucleic acid from FFPE, formalinized, fresh, or frozen pathologic specimens offers a culture-free approach to the detection and molecular characterization of MTB infection where the diagnosis relies on tissue examination in the absence of a culture isolate.
Special Instructions: Click here to print the Molecular Diagnostics requisition.

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