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Vanderbilt Pathology Laboratory Services

Test Directory



VZV DETECTION BY AMPLIFIED PROBE (VZD)

Department: Molecular Infectious Disease
Test Synonym(s): Varicella Zoster Virus, VZD, VZV DNA PCR
CPT Codes: 87798
Methodology: PCR (Polymerase Chain Reaction)
Reference Range: Not detected
Tube Type: CSF container - 1.0 mL
Specimen: CSF
Alternate Specimen: Blood, lesions/ulcers, and eye/vitreous fluid will be accepted as nonpreferred specimen types, and results will be reported with a disclaimer. Blood should be collected in a lavender top (EDTA) tube. Lesions/ulcer swabs should be submitted in Universal (viral) Transport Medium. Eye/vitreous fluid should be submitted in a sterile container (preferably) or Universal Transport Medium. Other specimen types require Medical Director approval; contact the lab prior to specimen collection for approval and collection instructions.
Pediatric Requirements: CSF container - 1.0 mL
Volume: 1.0 mL
Minimum Volume: 0.2 mL
Temperature: Room Temperature: same day, refrigerated: overnight, frozen: longer than overnight
Stability: Room temperature: same day, refrigerated: overnight, frozen: longer than overnight
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Monday, Wednesday and Friday
Times Performed: Once per day on Monday, Wednesday and Friday (batched)
TAT: 24 hours (Monday - Friday)
Significance: VZV is an important pathogen causing central nervous system infections, especially meningitis in HIV-1 infected patients. Prompt recognition of the presence of VZV is critical to the therapy initiation. Cell cultures have not been reliable for recovering this virus from cerebrospinal fluid (CSF). Detection of VZV DNA in CSF is now the gold standard for the laboratory diagnosis of central nervous system diseases caused by this virus.
Special Instructions: Other sources or samples may be acceptable for this test; please check with the lab, 615-936-6435.
Click here to print the Molecular Diagnostics requisition.

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