Vanderbilt Pathology Laboratory Services
Test Directory
TOBRAMYCIN LEVEL (TML)
Department: ToxicologyTest Synonym(s): TML, Nebcin, Tobrex
CPT Codes: 80200
Methodology: FPIA
Reference Range: Expected peak concentration: Adult 5-10 mcg/mL. Infant / peds: 2-4 mg/kg = 5-10 mcg/mL, 5-7 mg/kg = >15 mcg/mL. Target trough concentration: Adult: <0.5 mcg/mL. Infant / Peds: <2 mcg/mL. Potentially toxic trough: Adult: Q24 > 0.5 mcg/mL, Q8/Q12 > 2 mcg/mL. Peds: Q24 > 1 mcg/mL, Q8/Q12 > 2 mcg/mL. Infant: Q24 > 2 mcg/mL, Q8/Q12 > 2 mcg/mL.
Tube Type: Dark green top (Sodium Heparin)
Specimen: Blood
Alternate Specimen: Red top (no gel)
Pediatric Requirements: 1 dark green microtainer
Volume: 5 mL blood
Minimum Volume: 0.5 mL serum or plasma
Temperature: Refrigerated if not delivered to the lab immediately
Stability: Refrigerated: 2 days, frozen at -20 C for longer storage
Reasons for Rejection: Hemolysis, low volume
Days Performed: Daily
Times Performed: Continuous
TAT: Stat: 1 hour, routine: 2 hours
Special Instructions: Peak serum levels generally - draw 30 minutes after 30 min IV. Submit date/time IV start, date/time IV stop, peak collection time, dosage amount/interval. Trough serum levels generally - draw 30 minutes before next dose, following third dose. Provide date/time last dose charted, collection date/time, dosage amount/interval. Indicate if peak or trough specimen.
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