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Vanderbilt Pathology Laboratory Services

Test Directory



HIV - 1 & 2 ANTIBODY AND HIV 1 P24 ANTIGEN (HIV)

Department: Virology
Test Synonym(s): HIV, HIV ag/ab combo
Test Components: P24 antigen and HIV-1 & 2 abs
CPT Codes: 87389
Methodology: Chemiluminescence
Reference Range: Reactive, nonreactive
Tube Type: Red top (clot activator with gel)
Specimen: Serum
Alternate Specimen: Lavender top (EDTA) plasma
Pediatric Requirements: Red or lavender microtainer (1 mL) x 2
Volume: 500 uL (includes duplicate repeat and blot)
Minimum Volume: 200 uL (no repeat, no blot)
Temperature: Allow to clot, store refrigerated (2-8C)


Stability: Labile specimen. Keep refrigerated.
Reasons for Rejection: Same assay within 4 days, grossly hemolyzed, obvious microbial contamination, fluids other than serum/plasma
Days Performed: Monday - Friday
Times Performed: Morning
TAT: HIV: same day - 2 1/2 days; Western Blot; 2-4 days
Significance: Negative result does not rule out infection with HIV; patient may be seronegative during acute infection.
Special Instructions: HIV-2 p24 antigen and antibodies to HIV-1&2 are detected simultaneously in this assay. Group M and O HIV-1 strains are detected. It is not possible to distinguish positive signals generated from viral antigen and antibody. Repeatedly reactive specimens are confirmed by western blot assay. A negative result does not rule out infection with HIV; the patient may be negative during seroconversion. The performance of this assay has not been established for individuals younger than 2 years old.



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