HIV - 1 & 2 ANTIBODY AND HIV 1 P24 ANTIGEN (HIV)
: HIV, HIV ag/ab combo
: P24 antigen and HIV-1 & 2 abs
: Reactive, nonreactive
: Red top (clot activator with gel)
: Lavender top (EDTA) plasma
: Red or lavender microtainer (1 mL) x 2
: 500 uL (includes duplicate repeat and blot)
: 200 uL (no repeat, no blot)
: Allow to clot, store refrigerated (2-8°C)
: Labile specimen. Keep refrigerated.
Reasons for Rejection
: Same assay within 4 days, grossly hemolyzed, obvious microbial contamination, fluids other than serum/plasma
: Monday - Friday
: HIV: same day - 2 1/2 days; Western Blot; 2-4 days
: Negative result does not rule out infection with HIV; patient may be seronegative during acute infection.
: HIV-2 p24 antigen and antibodies to HIV-1&2 are detected simultaneously in this assay. Group M and O HIV-1 strains are detected. It is not possible to distinguish positive signals generated from viral antigen and antibody. Repeatedly reactive specimens are confirmed by western blot assay. A negative result does not rule out infection with HIV; the patient may be negative during seroconversion. The performance of this assay has not been established for individuals younger than 2 years old.
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