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Vanderbilt Pathology Laboratory Services

Test Directory



INFLUENZA A & B ANTIGEN DETECTION (FLG)

Department: Virology
Test Synonym(s): Flu antigen, FLG, Rapid flu antigen
CPT Codes: 87400
Methodology: Immunochromatographic assay; negative antigen on inpatient or employee reflexes to rapid respiratory virus culture (a.k.a, "shell vial" culture)
Reference Range: Negative, Positive, Indeterminate. A negative result does not rule out infection with influenza.
Tube Type: Nasopharyngeal/nasal wash or aspirate in sterile leakproof container or nasopharyngeal swab in 3 mL Universal Transport Medium (swab is packaged with medium).
Specimen: Material from respiratory source only; Nasopharyngeal (NP) swab/aspirate/wash or nasal wash preferred
Alternate Specimen: Dacron/Rayon nasopharyngeal swab in 3 mL sterile saline.
Volume: 3 mL Universal Transport Medium or sterile saline
Minimum Volume: 1 mL Universal Transport Medium or sterile saline
Temperature: Store refrigerated (2-8 C), do not freeze, transport on wet ice
Stability: Samples older than 24 hours will receive comment
Reasons for Rejection: Non-respiratory source, calcium alginate or wooden-shaft swab, dry swab
Days Performed: Daily
Times Performed: Continuous; Processed ASAP upon request
TAT: 30 minutes - 1 hour
Special Instructions: Send to lab immediately - put swab in UTM - No wooden shaft or calcium alginate tip swabs. Add universal (viral) transport medium (UTM) to respiratory trap. Specify source. Negative anitgen results on inpatients and employees confirmed by rapid shell vial culture.

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