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Vanderbilt Pathology Laboratory Services

Test Directory



INFLUENZA A & B ANTIGEN DETECTION (FLG)

Department: Virology
Test Synonym(s): Flu antigen, FLG, Rapid flu antigen
CPT Codes: 87400
Methodology: Immunochromatographic assay; negative antigen on inpatient or employee reflexes to rapid respiratory virus culture (a.k.a, "shell vial" culture)
Reference Range: Negative
Tube Type: Wash, aspirate in sterile leakproof container; flocked swab preferred but dacron/rayon swab accepted in universal transport medium, 3 mL or 1-2 mL saline
Specimen: Material from respiratory source only; Nasopharyngeal (NP) swab or nasal swab/aspirate/wash preferred
Alternate Specimen: Dacron/Rayon nasopharyngeal swab in 3 mL sterile saline.
Volume: 3 mL Universal Transport Medium or sterile saline
Minimum Volume: 1 mL Universal Transport Medium or sterile saline
Temperature: Store refrigerated (2-8 C), do not freeze, transport on wet ice
Stability: Samples older than 24 hours will receive comment
Reasons for Rejection: Non-respiratory source, calcium alginate or wooden-shaft swab, dry swab
Days Performed: Daily
Times Performed: Continuous; Processed ASAP upon request
TAT: 30 minutes - 1 hour
Special Instructions: Send to lab immediately - put swab in UTM - No wooden shaft or calcium alginate tip swabs. Add universal (viral) transport medium (UTM) to respiratory trap. Specify source. Negative antigen results on inpatients confirmed by rapid shell vial culture. A negative result does not rule out infection with influenza.

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