CULTURE SPUTUM AFB (SPA)
: SPA, Acid fast bacilli, Sputum culture
: AFB culture and stain
: 87116: culture, 87206: stain, 87015: concentration
: Culture and fluorochrome stain
: Negative for acid fast bacilli
: Sterile sputum container
: Sputum (No 24 hour specimens)
: 5-10 mL
: Room temperature
: Send to lab immediately
Reasons for Rejection
: Specimen not received in appropriate sterile container, specimen received on a swab, specimen leaking, excessive delay in transport, or 24 hour specimen.
: AFB smear: 24 hours. Culture: 6 weeks for negative culture. Varies for positive culture
: It is recommended that 3 sputum specimens should be collected for acid-fast smears and culture in patients with clinical and chest x-ray findings compatible with tuberculosis. These three samples should be collected at 8-24 hour intervals (24 hours when possible) and should include at least one first morning specimen. Specimens must be delivered to the laboratory promptly; specimens that cannot be processed within one hour of the time of collection should be refrigerated during transport to and storage in the laboratory prior to processing. This will decrease overgrowth with contaminating organisms likely to be present.
Fresh deep cough specimen - early morning specimen is best. No 24 hour specimens. AFB smear included with culture and has a separate charge. There will be separate charges for organism identification for positive cultures. AFB susceptibility testing is sent out to a reference laboratory. Mycobacterium tuberculosis susceptibility testing is done automatically by the TN State Lab. Susceptibility testing for other Mycobacterium species must be requested; these tests are sent to a reference laboratory.
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