CULTURE SPUTUM AFB (SPA)
: SPA, Acid fast bacilli, Sputum culture
: AFB culture and stain
: 87116: culture, 87206: stain, 87015: concentration
: Culture and fluorochrome stain
: Negative for acid fast bacilli
: Sterile sputum container
: Sputum (No 24 hour specimens)
: 5-10 mL
: Room temperature
: Send to lab immediately
Reasons for Rejection
: Specimen not received in appropriate sterile container, specimen received on a swab, specimen leaking, excessive delay in transport, or 24 hour specimen.
: AFB smear: 24 hours. Culture: 6 weeks for negative culture. Varies for positive culture
: Fresh deep cough specimen - early morning specimen is best. No 24 hour specimens. AFB smear included with culture and has a separate charge. There will be separate charges for organism identification for positive cultures. AFB susceptibility testing is sent out to a reference laboratory. Mycobacterium tuberculosis susceptibility testing is done automatically by the TN State Lab. Susceptibility testing for other Mycobacterium species must be requested; these tests are sent to a reference laboratory.
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