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Vanderbilt Pathology Laboratory Services

Test Directory



HERPES SIMPLEX CULTURE (HVC)

Department: Virology
Test Synonym(s): HVC, HSV
Test Components: Herpes simplex culture
CPT Codes: 87252
Methodology: Tissue culture
Reference Range: No virus isolated
Tube Type: Liquids in sterile leakproof container with NO ADDITIVE; tissue or swab in universal (viral) transport media,3ml
Specimen: Vesicular material in universal (viral) transport medium (UTM), tissue, swab, wash in universal transport medium (UTM), liquids. NOT PERFORMED ON CSF without prior pathologist approval (PCR is most sensitive assay for detection of HSV in CSF).
Alternate Specimen: Blood requires consult with the lab.
Pediatric Requirements: 1 - 2 mL urine
Temperature: Store refrigerated (2-8 C), do not freeze, transport on wet ice


Stability: Labile specimen. Keep refrigerated.
Reasons for Rejection: Collected more than 1 day prior to receipt in lab, calcium alginate or wooden-shaft swab, dry swab
Days Performed: Daily
Times Performed: Monday - Friday: 6:30 am to 11:00 pm, Saturday and Sunday 7:00 am to 3:30 pm.
TAT: 1 - 10 days
Special Instructions: Send to Virology immediately on wet ice. Collect fluid from vesicles with a tuberculin syringe. Rinse fluid into viral transport medium. Vigorously swab the base of the lesions add to viral transport medium. No wooden shaft or calcium alginate tip swabs. Additional tests/charges may be added as appropriate.

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