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Vanderbilt Pathology Laboratory Services

Test Directory



JAK 2 (JK2)

Department: Molecular Diagnostics
Test Synonym(s): JK2, Janus-kinase 2 gene, V617F
CPT Codes: 81270
Methodology: Allele Specific Detection of Mutation V617F by PCR and evaluated by capillary electrophoresis
Tube Type: Lavender top (EDTA)
Specimen: Whole blood, bone marrow or purified DNA (1g)
Alternate Specimen: Yellow top (ACDH) for whole blood or bone marrow
Pediatric Requirements: 0.5 mL whole blood or bone marrow
Volume: 4 mL whole blood or bone marrow
Minimum Volume: 0.5 mL whole blood or bone marrow
Temperature: EDTA and ACDH: Room temperature or refrigerated, purified DNA: refrigerate or freeze.
Stability: EDTA and ACDH tubes - Room temperature: 2 days Refrigerated: 3 days; Purified DNA - indefinitely @ -70C;
Reasons for Rejection: Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
Days Performed: Wednesday
Times Performed: Batched
TAT: 7 - 10 days
Significance: Associated with Myeloproliferative disorders
Special Instructions: Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission or if patient has received a transfusion in the prior 2 weeks.
Click here to print the Molecular Diagnostics requisition.

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