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Vanderbilt Pathology Laboratory Services

Test Directory



ANTITHROMBIN ANTIGEN (ATG)

Department: Coagulation
Test Synonym(s): ATG, AT3 Antigen
CPT Codes: 85301
Methodology: Immunoturbidimetric
Reference Range: 85 - 135%
Tube Type: Light blue top (Sodium Citrate)- 2.7 mL
Specimen: Citrated plasma - platelet poor. This sample requires double centrifugation if sending a plasma aliquot

Pediatric Requirements: Peds: 2.7 mL; Neonates: 1.8 mL
Volume: 1.0 mL
Minimum Volume: 1.0 mL
Temperature: Room temperature or send as frozen plasma aliquot if sample will not be received into the lab within 8 hours



Stability: Refrigerated: 8 hours, frozen at -70 C: one month
Reasons for Rejection: Clotted sample, incorrectly filled tube, grossly lipemic plasma, sample sent as whole blood > 8 hours old



Days Performed: Monday - Friday
Times Performed: 8:00 a.m. - 4:00 p.m.
TAT: 4 days
Significance: Cloudy plasma may lead to an underestimation of the antithrombin antigen level.



Special Instructions: Indwelling line should be flushed with saline and 5.0 mL of blood discarded before collecting samples for coagulation assays. Tube must be full and not clotted. Deliver to lab immediately. Samples received from an outside lab must be aliquoted and frozen. Samples aliquoted for shipping must be double centrifuged prior to freezing. Grossly hemolyzed, icteric or lipemic samples may be rejected.


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