Gould Lab

Reagent information

TAP tag information

Our S. pombe tandem affinity purification (TAP) protocol was adapted from the Seraphin lab S. cerevisiae protocol (see reference and website below). Click here for the pdf version of our protocol.

Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology17: 1030-1032.

http://www.embl-eidelberg.de/ExternalInfo/seraphin/TAP.html

Our TAP Tag vector schematics and sequences:

2X PA CTAP in pFA6a | schematic | sequence | (html) | (pdf)
4X PA CTAP in pFA6a | schematic | sequence | (html) | (pdf)
CTAP Vector (html) | (pdf)
NTAP Cassette | schematic |sequence |(html) | (pdf)
NTAP TAG | sequence | (html) | (pdf)
NTAP Vector| sequence | (html) | (pdf)
pREP-NTAP Vector | schematic | (html) | (pdf)
3HA 2XPA CTAP pFS209 (from N. Rhind)

YFP-CFP dual cassette

schematic (pdf)
sequence (html) | (pdf)

Vanderbilt University Medical Center, 21st Avenue South, Nashville, TN 37232

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