Our protocol for S. pombe was adapted from the Seraphin Lab protocol used for S. cerevisiae.
Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology17: 1030-1032.
Their web site: http://www.embl-eidelberg.de/ExternalInfo/seraphin/TAP.html
Click here to open our S. pombeTAP Tag protocol in Adobe Acrobat .PDF format
Our TAP Tag vector schematics and sequences:
Schematics:
- 2X PA CTAP 4X PA CTAP 2X PA NTAP Cassette pREP-NTAP Vector
- 3HA 2XPA CTAP pFS209 (from N. Rhind)
Sequences:
* note: to work with html sequences, first copy the sequence, then paste into your sequence editor of choice (Vector NTI, MacVector, Word, etc).
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