Imaging quantification has potential advantages over conventional pathologist-based manual scoring, such as automation and the ability to quantify rapidly using a continuous scale, which may overcome inaccuracies in distinguishing between 0 and 1+, 2+, and 3+ in the conventional four-point scale. Further applications of this system in kidney research include quantitative image analysis of immunostaining:
1) Based on the distinctive density and color of immunoreactive product in video images, the number, size, density, and position of stained cells are quantified using the BIOQUANT true-color windows system equipped with digital stage encoders that allow high-magnification images to be mapped to global coordinates throughout the whole kidney. Sections from at least three regions of each kidney are routinely analyzed. In addition, topographic distribution of immunoreactive products in kidney can also be determined.
2) Quantification of glomerular number and measurement of glomerular diameter.
3) Measurement of lumen diameters and the height of epithelial cells of nephron segments of interest.
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Last updated on 2007-02-06 Moderated by Ming-Zhi Zhang