.

James May Lab

Elizabeth Meredith

I hail from East Tennessee, Johnson City to be specific. In 2008, I received by BA in Biology and Exercise Science from Transylvania University in Lexington, KY. I began graduate school at Vanderbilt in 2008.

I am working to determine how the Sodium-dependent Vitamin C Transporter 2 (SVCT2) is regulated in the brain throughout development and in adulthood. SVCT2 is one of two ascorbic acid (AA; vitamin C) transporters and the only AA transporter expressed in the brain. Brain AA levels have been shown to decrease during development in mice, reaching a steady-state level around post natal day 30. I am investigating how the expression levels of SVCT2 vary during development and whether SVCT2 is regulated by AA or via some other mechanisms. This is important in humans as we cannot make our own AA like most animals and must acquire it through diet and different AA intakes lead to varying tissue levels of AA. To understand how SVCT2 is regulated by AA in vivo I utilize Gulo(-/-) mice, which are unable to synthesize their own AA. These mice can be supplemented with varying levels of AA to alter their tissue AA levels accordingly. I use real-time PCR and Western immunoblotting techniques to measure SVCT2 mRNA and protein levels, to further characterize this transporter.

Recent publications:
Harrison FE, Meredith ME, Dawes SM, Saskowski JL, May JM. (2010) Low ascorbic acid and increased oxidative stress in gulo(-/-) mice during development. Brain Res. 1349:143-52.
Harrison FE, Dawes SM, Meredith ME, Babaev VR, Li L, May JM. (2010) Low vitamin C and increased oxidative stress and cell death in mice that lack the sodium-dependent vitamin C transporter SVCT2. Free Radic Biol Med. 49(5):821-9.
Berglund ED, Lee-Young RS, Lustig DG, Lynes SE, Donahue EP, Camacho RC, Meredith ME, Magnuson MA, Charron MJ, Wasserman DH. (2009) Hepatic energy state is regulated by glucagon receptor signaling in mice. J Clin Invest. 119(8):2412-22.
 

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