Department of Pathology, Microbiology, and Immunology



   Andries Zijlstra, Ph.D.



Associate Professor

Dept of Pathology, Microbiology, and Immunology


  Contact Information



Office Location:

MCN C-2102C

Phone: 615-322-3295
E-mail:  Andries.Zijlstra@vanderbilt.edu

Website: https://my.vanderbilt.edu/zijlstra/

Campus Mail address:

Pathology, Microbiology and Immunology-3rd Fl

C-3321 MCN (2561)

US Mailing address:

Vanderbilt University School of Medicine

Dept of Pathology, Microbiology and Immunology-3rd Fl

MCN C-3321

Nashville, TN 37232-2561






Ph.D., Washington State
B.S., Washington State



Research Keywords



Cancer, Metastasis, Dissemination, Clinical Translation, Adhesion, Invasion, Migration, Tetraspanin, surface protein organization, Integrin, Tumor Biology



Research Description



Cancer Metastasis is the primary cause of cancer-related deaths. Research in the laboratory is dedicated to understanding the molecular biology of cancer metastasis and translating this knowledge to clinical application.

The research program is based on three central themes: A) Investigating the molecular mechanisms of tumor cell migration, B) Evaluating and validating these mechanisms in the patient population, and C) developing research and clinical tools to advance our studies.

The laboratory pursues three primary research objectives: 1) Characterization of the molecular mechanism of migration regulated by the tetraspanin CD151, 2) Identification and characterization of the metastatic cell population within a primary tumor, and 3) clinical implementation of molecular markers of migration as biomarkers of tumor progression and metastasis.

Specific mechanistic studies include:???The regulation of migration by tetraspanins: Among the molecular regulators of motility, we have found the tetraspanin CD151 to be a particularly critical component of metastasis. Interfering with its function through antibody binding inhibits extracellular matrix mediated migration and blocks >95% of the tumor cell dissemination in a spontaneous metastasis model. Using a newly-established in vivo motility assay, we have been able to demonstrate that altering the function of CD151 results in complete inhibition of in vivo motility for several tumor cell types. Ongoing studies try to determine the molecular mechanism by which this regulation of migration occurs.

Specific translational studies include:???Molecular mechanisms of migration contribute to metastasis and subsequently thought to be central to the cancer progression poor clinical outcome for cancer patients. We have developed a series of preclinical tests that determine the status of pro-migratory mechanisms within the tumor. Using this technology it becomes possible to diagnose patients with aggressive disease, predict clinical outcome, and possible anticipate treatment response. Ongoing studies are expanding biomarker studies to a variety of cancers, including renal, bladder, prostate, lung, and breast cancer.

Specific technological advances:???We have developed novel optical imaging strategies using fluorescent and luminescent proteins to visualize cell behavior along the metastatic cascade in vivo. In addition to visualizing the temporal and spatial dymics of metastasis, we implement imaging and biochemical strategies to assess quantitatively how metastasis is impacted by specific mechanisms of motility.






Hansen, AG, Arnold, SA, Jiang, M, Palmer, TD, Ketova, T, Merkel, A, Pickup, M, Samaras, S, Shyr, Y, Moses, HL, Hayward, SW, Sterling, JA, Zijlstra, A. ALCAM/CD166 is a TGF-??-responsive marker and functional regulator of prostate cancer metastasis to bone. Cancer Res, 74(5), 1404-15, 2014

Irvin, MW, Zijlstra, A, Wikswo, JP, Pozzi, A. Techniques and assays for the study of angiogenesis. Exp Biol Med (Maywood), 2014

Madamanchi, A, Zijlstra, A, Zutter, MM. Flipping the switch: integrin switching provides metastatic competence. Sci Signal, 7(318), pe9, 2014

Palmer, TD, Mart??nez, CH, Vasquez, C, Hebron, KE, Jones-Paris, C, Arnold, SA, Chan, SM, Chalasani, V, Gomez-Lemus, JA, Williams, AK, Chin, JL, Giannico, GA, Ketova, T, Lewis, JD, Zijlstra, A. Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering and is a clinical indicator of prostate cancer progression. Cancer Res, 74(1), 173-87, 2014

Hansen, AG, Freeman, TJ, Arnold, SA, Starchenko, A, Jones-Paris, CR, Gilger, MA, Washington, MK, Fan, KH, Shyr, Y, Beauchamp, RD, Zijlstra, A. Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome. Cancer Res, 73(10), 2955-64, 2013

Campbell, JP, Karolak, MR, Ma, Y, Perrien, DS, Masood-Campbell, SK, Penner, NL, Munoz, SA, Zijlstra, A, Yang, X, Sterling, JA, Elefteriou, F. Stimulation of host bone marrow stromal cells by sympathetic nerves promotes breast cancer bone metastasis in mice. PLoS Biol, 10(7), e1001363, 2012

Matise, LA, Palmer, TD, Ashby, WJ, Nashabi, A, Chytil, A, Aakre, M, Pickup, MW, Gorska, AE, Zijlstra, A, Moses, HL. Lack of transforming growth factor-?? signaling promotes collective cancer cell invasion through tumor-stromal crosstalk. Breast Cancer Res, 14(4), R98, 2012

Ashby, WJ, Wikswo, JP, Zijlstra, A. Magnetically attachable stencils and the non-destructive analysis of the contribution made by the underlying matrix to cell migration. Biomaterials, 33(33), 8189-203, 2012

Ashby, WJ, Zijlstra, A. Established and novel methods of interrogating two-dimensional cell migration. Integr Biol (Camb), 4(11), 1338-50, 2012

Ramirez, NE, Zhang, Z, Madamanchi, A, Boyd, KL, O''Rear, LD, Nashabi, A, Li, Z, Dupont, WD, Zijlstra, A, Zutter, MM. The I?a??I?a?? integrin is a metastasis suppressor in mouse models and human cancer. J Clin Invest, 121(1), 226-37, 2011

Gao, Y, Majumdar, D, Jovanovic, B, Shaifer, C, Lin, PC, Zijlstra, A, Webb, DJ, Li, D. A versatile valve-enabled microfluidic cell co-culture platform and demonstration of its applications to neurobiology and cancer biology. Biomed Microdevices, 13(3), 539-48, 2011

Palmer, TD, Lewis, J, Zijlstra, A. Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model. J Vis Exp(51), 2011

Palmer, TD, Ashby, WJ, Lewis, JD, Zijlstra, A. Targeting tumor cell motility to prevent metastasis. Adv Drug Deliv Rev, 63(8), 568-81, 2011

Andries Zijlstra. Tetraspanins in Cancer. In: Cell-Extracellular Matrix Interactions in Cancer, Springer Science + Business Media(1st Ed.), 217-244, 2010

Zijlstra, A. Launching an academic research career. Dis Model Mech, 2(11-12), 526-30, 2009

Zijlstra, A, Lewis, J, Degryse, B, Stuhlmann, H, Quigley, JP. The inhibition of tumor cell intravasation and subsequent metastasis via regulation of in vivo tumor cell motility by the tetraspanin CD151. Cancer Cell, 13(3), 221-34, 2008

F??ral, CC, Zijlstra, A, Tkachenko, E, Prager, G, Gardel, ML, Slepak, M, Ginsberg, MH. CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling. J Cell Biol, 178(4), 701-11, 2007

Zijlstra, A, Seandel, M, Kupriyanova, TA, Partridge, JJ, Madsen, MA, Hahn-Dantona, EA, Quigley, JP, Deryugina, EI. Proangiogenic role of neutrophil-like inflammatory heterophils during neovascularization induced by growth factors and human tumor cells. Blood, 107(1), 317-27, 2006

Lewis, JD, Destito, G, Zijlstra, A, Gonzalez, MJ, Quigley, JP, Manchester, M, Stuhlmann, H. Viral nanoparticles as tools for intravital vascular imaging. Nat Med, 12(3), 354-60, 2006

Zijlstra, A, Quigley, JP. The DARC side of metastasis: shining a light on KAI1-mediated metastasis suppression in the vascular tunnel. Cancer Cell, 10(3), 177-8, 2006

Wilson, S, Greer, B, Hooper, J, Zijlstra, A, Walker, B, Quigley, J, Hawthorne, S. The membrane-anchored serine protease, TMPRSS2, activates PAR-2 in prostate cancer cells. Biochem J, 388(Pt 3), 967-72, 2005

Blancafort, P, Chen, EI, Gonzalez, B, Bergquist, S, Zijlstra, A, Guthy, D, Brachat, A, Brakenhoff, RH, Quigley, JP, Erdmann, D, Barbas, CF. Genetic reprogramming of tumor cells by zinc finger transcription factors. Proc Natl Acad Sci U S A, 102(33), 11716-21, 2005

Deryugina, EI, Zijlstra, A, Partridge, JJ, Kupriyanova, TA, Madsen, MA, Papagiannakopoulos, T, Quigley, JP. Unexpected effect of matrix metalloproteinase down-regulation on vascular intravasation and metastasis of human fibrosarcoma cells selected in vivo for high rates of dissemination. Cancer Res, 65(23), 10959-69, 2005

Zijlstra, A, Aimes, RT, Zhu, D, Regazzoni, K, Kupriyanova, T, Seandel, M, Deryugina, EI, Quigley, JP. Collagenolysis-dependent angiogenesis mediated by matrix metalloproteinase-13 (collagenase-3). J Biol Chem, 279(26), 27633-45, 2004

Aimes, RT, Zijlstra, A, Hooper, JD, Ogbourne, SM, Sit, ML, Fuchs, S, Gotley, DC, Quigley, JP, Antalis, TM. Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thromb Haemost, 89(3), 561-72, 2003

Hooper, JD, Zijlstra, A, Aimes, RT, Liang, H, Claassen, GF, Tarin, D, Testa, JE, Quigley, JP. Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene, 22(12), 1783-94, 2003

Zijlstra, A, Testa, JE, Quigley, JP. Targeting the proteome/epitome, implementation of subtractive immunization. Biochem Biophys Res Commun, 303(3), 733-44, 2003

Zijlstra, A, Mellor, R, Panzarella, G, Aimes, RT, Hooper, JD, Marchenko, ND, Quigley, JP. A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction. Cancer Res, 62(23), 7083-92, 2002

Balza, E, Castellani, P, Zijlstra, A, Neri, D, Zardi, L, Siri, A. Lack of specificity of endoglin expression for tumor blood vessels. Int J Cancer, 94(4), 579-85, 2001

Zijlstra, A, McCabe, NR, Schelling, ME. Expression and assembly of the angiogenic marker B-fibronectin by endothelial cells in vitro: regulation by confluency. Angiogenesis, 3(1), 77-87, 1999

Zijlstra, A, Schelling, ME. Detection of multiple fibronectin isoforms in fetal fibroblast monolayer culture: a novel method for the qualitative and quantitative detection of multiple antigens. Histochem Cell Biol, 111(2), 163-9, 1999