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Education

Informing and inspiring patients and professionals about personalized care, research and training opportunities in kidney disease

Program Summary

Day 1: Basic Techniques

7:00-8:00am

Orientation with breakfast (Mark de Caestecker and Suzanne Galford)

8:00-9:00am

Mouse Practices Lecture (Scott Bury and Avi Barlatier)
Basic information about the mouse, basic handling and restraint
1) Working with IACUC and AVMA euthanasia guidelines
2) Discussion and training videos on: a) Compound administration (e.g. IP, SQ, IV tail vein; saphenous vein and retro-orbital); b) Blood collection (e.g. tail nick/prick, submandibular, saphenous vein)

9:00-10:00am

Mouse Anesthesia and Surgery Lecture (Erin Yu)
Anesthesia and monitoring during anesthesia
Post-procedural monitoring
Discuss osmotic mini-pumps for SQ, IP and IV administration

10:15-10:30am

Break

10:30am-4:30pm

Hands on training (Instructors: Avi Barlatier, Amanda Darbyshire, Kelsie Finney, Logan France, Amy MacKenzie, Tai Oatess, and Erin Yu)

10:30am-12:30pm

Mouse Procedures 1
Demonstrate and practice bleeding techniques including tail vein (nick/prick), submandibular, saphenous as well as thoracotomy and cardiac puncture as a terminal procedure. Anesthesia monitoring will be practiced throughout this training. Anesthetized mice will also be euthanized via cervical dislocation at the end of the session.

12:30-1:00pm

Lunch

1:00-3:00pm

Mouse Procedures 2
Demonstrate and practice IV injection by tail vein. Demonstrate and practice oral gavage and perform blind cardiac puncture in anesthetized mice. Cervical dislocation will be performed at the end of the session.

3:00-3:15pm

Break - Suture video

3:15-4:30pm

Mouse Procedures 3
Demonstrate and practice simple interrupted suture pattern. Participants will practice on SIMs suture boards and then perform a SC osmotic pump implant in mice to include skin closure. This training will address surgical preparation, surgery, skin closure and anesthesia monitoring. Cervical dislocation of the mice will be performed at the end of the session.

Day 2: Mouse Renal Pathology and Tissue Preparation

Morning Session

Lectures

7:30-8:00am

Coffee and Breakfast

8:00-8:45am

Mouse Renal Pathology-Part 1 (Agnes Fogo)
Overview of mouse renal pathology, with specific examples illustrating IR-AKI, UUO and 5/6 nephrectomy models
Tissue harvesting and fixation methods, mounting and processing

8:45-9:30am

Overview of Mouse Pathology (Kelli Boyd)
Pathology phenotyping
Issues regarding mouse strain, age and health status
Tissue collection and fixation techniques; Comparative pathology

9:30-10:15am

Renal Function Tests in Mice (Mark de Caestecker)
Kidney structure and function
Venesection, serum creatinine and BUN, GFR, urinary albumin

10:15-10:30am

Break

10:30-11:15am

Mouse Renal Pathology-Part 2 (Haichun Yang)
Basic staining (PAS/Trichrome/Sirius Red)
Immunofluorescence staining tips

11:15-12:00pm

Mouse Renal Pathology-Part 3 (Haichun Yang)
Quantitative histological analysis

12:00-12:30pm

Lunch

12:30-1:30pm

Mouse Renal Pathology-Part 4 (Haichun Yang)
Mouse kidney pathology-virtual practice (Aperio Image Scope software)

2:00-5:00pm

Hands on Training

2:00-4:00pm

Perfusion fixation of Kidneys (Kelli Boyd and Ellen Donnert)
Demonstrate and practice perfusion fixation and preparation

4:00-4:30pm

Harvesting Kidneys (Haichun Yang, Ellen Donnert and Kelli Boyd)
Demonstrate and practice normal, UUO and 5/6 nephrectomies

Day 3: Acute Kidney Injury

Morning Session

Lectures

7:30-8:00am

Coffee and Breakfast

8:00-8:45am

Overview of AKI models-Part 1 (Craig Brooks)
Rodent models pros and cons (IR/toxin/sepsis/genetic)
Modeling human disease: what is known and what is unknown?

8:45-9:30am

Mouse models of AKI-Part 2 (Mark de Caestecker)
IR-AKI and toxic models, strain and gender effects, modeling humans

9:30-9:45am

Break

9:45-10:30am

Mouse models of AKI-Part 3 (Jonathan Street, NIDDK)
Models of sepsis-induced AKI in rodents, pros and cons
The Cecal Ligation and Perforation (CLP) model

10:30am-6:00pm

Hands on Training

10:30-12:30pm

Cecal Ligation and Perforation model (Jonathan Street)
Demonstrate and individual practice of mouse CLP

12:30-1:00pm

Lunch

1:00-1:30pm

Videos of afternoon practices
Unilateral Ureteric Obstruction
Ischemia reperfusion injury (dorsal approach)

1:30-3:30pm

Unilateral ureteric obstruction (Leslie Gewin, Haichun Yang and Rachel Delgado)
Demonstrate and individual practice unilateral ureteric obstruction

3:30-3:45pm

Break

3:45-6:30pm Ischemia reperfusion injury of the kidney (dorsal approach) (Jian-Chun Chen, Haichun Yang, Charlene Finney and Lauren Scarfe)
Demonstrate and individual practice of dorsal renal IR surgery

Day 4: Chronic Kidney Injury

Morning Session

Lectures

7:30-8:00am

Coffee and Breakfast

8:00-8:45am

Overview of CKD models (Roy Zent)
Rodent models of CKD pros and cons
Modeling human disease: what is known and what is unknown?

8:45-9:30am

CKD models in mice (Leslie Gewin)
Toxin and genetic
Surgical models (include details about 5/6 nephrectomy)
Pros and cons

9:30-10:15am

Diabetic models in mice (Ambra Pozzi)
Pro and cons of different diabetic nephropathy models
Modeling human disease

10:15-10:30am

Break

10:30-11:00am

Renal Biopsy Video
Renal Biopsy

11:00-12:00pm

Renal biopsy (Haichun Yang)
Demonstrate and individual practice renal biopsy

12:00-12:30pm

Lunch

12:30-1:00pm

Videos and discussion of afternoon practice
5/6 nephrectomy-polectomy and cautery/arterial ligation methods

1:00-6:00pm

Hands on training (Yan Su, Haichun Yang and Leslie Gewin)

1:00-6:00pm

5/6 nephrectomy (nephrectomy and polectomy method)
Demonstrate 5/6 nephrectomy

6:30pm till late

Night out on the town!

Day 5: Review and Feedback

Morning Session

8:30-9:00am

Coffee and Breakfast

9:00-12:00pm

Review and demonstration of Transcutaneous GFR measurement (Lauren Scarfe and Daniel Schock-Kusch)
Short talk followed by video
Two demonstrations in normal mice and mice after 5/6 nephrectomies

12:00-12:30pm

Lunch

12:30-1:00pm

Review training videos and discuss specific surgical techniques
UUO, 5/6 nephrectomy, IR surgery and renal biopsy
Ischemia reperfusion injury (ventral approach)
Discuss afternoon practice options

1:00pm-4:00pm

Hands on training

1:00pm-4:00pm

Additional supervised practice surgical AKI and CKD models(Jian-Chun Chen, Yan Su, Haichun Yang, Rachel Delgado, Charlene Finney, and Lauren Scarfe)
IR-AKI, UUO, 5/6 nephrectomy, CLP and renal biopsy

4:00-5:00pm

Feedback and closing remarks